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huvecs  (BioMimetic Therapeutics)
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BioMimetic Therapeutics huvecs
Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with <t>HUVECs,</t> accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of <t>the</t> <t>microgel</t> assembly process (reproduced with permission from , ).
Huvecs, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary human coronary artery vascular endothelial cells  (Lonza)
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Lonza primary human coronary artery vascular endothelial cells
Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with <t>HUVECs,</t> accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of <t>the</t> <t>microgel</t> assembly process (reproduced with permission from , ).
Primary Human Coronary Artery Vascular Endothelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exs from human umbilical vascular endothelial cells (huvecs)  (NanoSight ltd)
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NanoSight ltd exs from human umbilical vascular endothelial cells (huvecs)
Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with <t>HUVECs,</t> accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of <t>the</t> <t>microgel</t> assembly process (reproduced with permission from , ).
Exs From Human Umbilical Vascular Endothelial Cells (Huvecs), supplied by NanoSight ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvec (human umbilical vascular endothelial cells  (Becton Dickinson)
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Becton Dickinson huvec (human umbilical vascular endothelial cells
Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with <t>HUVECs,</t> accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of <t>the</t> <t>microgel</t> assembly process (reproduced with permission from , ).
Huvec (Human Umbilical Vascular Endothelial Cells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human primary vascular endothelial cells lonza verviers sprl  (Lonza)
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Lonza human primary vascular endothelial cells lonza verviers sprl
Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with <t>HUVECs,</t> accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of <t>the</t> <t>microgel</t> assembly process (reproduced with permission from , ).
Human Primary Vascular Endothelial Cells Lonza Verviers Sprl, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with HUVECs, accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of the microgel assembly process (reproduced with permission from , ).

Journal: Journal of Pharmaceutical Analysis

Article Title: 3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

doi: 10.1016/j.jpha.2018.08.005

Figure Lengend Snippet: Micromodule assembly: (A) collagen solution loaded with human hepatoma (HepG2) cells was gelled into ethylene oxide tube at 37°C for 30 min, the tube was then segmented into 2-mm length, and the collagen modules were collected after rotating in a centrifuge. Then HepG2 cell loaded modules were seeded with HUVECs, accumulated into a larger tube, and perfused with medium or blood, (B) light micrographic image of a collagen–HepG2 module without HUVECs, (C) confocal microscopic image of vascular endothelial (VE)-cadherin-stained module showing a confluent layer of HUVECs around the outer surface of module after 7 days of culture, (D) perfusion of a modular construct in a large tube with phosphate buffered saline (PBS), (E) confocal microscopic image of a collagen–HepG2–HUVEC module after 7 days of culture with HepG2 cells labeled with a Vybrant™ CFDA SE cell tracer kit, and (F) schematic diagram of the microgel assembly process (reproduced with permission from , ).

Article Snippet: In particular, SMCs and HUVECs were incorporated in the outer and inner layer of the microgel to form a biomimetic 3D vasculature.

Techniques: Staining, Construct, Saline, Labeling